不同毛色山羊皮肤组织Agouti基因剪接体类型研究

doi:10.11843/j.issn.0366-6964.2015.11.004

Abstract

Abstract:

The objective of this study was to investigate the variant types of goat Agouti gene spliceosome in goat skin with different coat color.The sequences and mRNA of goat Agouti gene were obtained and analyzed from goat skin with different coat color by PCR and 5′RACE methods，respectively.The 27 793 and 27 858 bp of partial Agouti gene sequences were obtained from white and black goat skin，respectively.Four fragments，88，180，81 and 79 bp，in which the 180，81 and 79 bp were first detected in goat，were matched with the sequences of sheep mRNA and corresponded with the length of exon 1 spliceosome obtained by 5′RACE.The 5 types（It，It′，IA，2C and IE） of spliceosome were detected in goat skin with white coat color，and only 2 types（It and It′） were found in goat skin with black coat color.There was length variation within and among spliceosomes.The result indicate that the IA，2C and IE might be related with the formation of goat coat color.

CLC Number:

S827
S813.3

ZHANG Tian，LI Xiang-long，ZHOU Rong-yan，LI Lan-hui. Study on Agouti Spliceosome Types in Goat Skin with Different Coat Color[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(11): 1934-1943.

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Study on Agouti Spliceosome Types in Goat Skin with Different Coat Color

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